Sets up PROTON8, CARBON, DEPT135,COSY,HSQC,HMBC in one clickġD 19F with 90° excitation and 1H decoupling during acquisition. Indirect TD fixed at 200 increments, default mixing time D9 = 0.045 sec. Each 13C resonance is coupled to the 1H covalently attached AND the 1H resonances in its 1H-1H spin-coupled network excellent for assigning 1H peaks overlapped in 1H, COSY, and TOCSY spectra. Gives good data on 10 mg samples in the time specified.ĢD 1H-1H TOtal COrrelation SpectroscopY with DIPSI spin lock for detecting peaks in the same network of 1H-1H coupled spins, indirect TD fixed at 128 increments, default mixing time D9 = 0.08 sec that can be changed by user.ĢD 1H-13C correlation combined with TOCSY with 1H direct detection axis, 13C indirect detection. With positive diagonal peaks, positive crosspeaks indicate NOE contacts, and negative crosspeaks indicate chemical exchange. Also known as the “EASY-ROESY”, this “jump-symmetrized” ROESY reduces TOCSY artifacts and improves sensitivity. Gives good data on 10 mg samples in the time specified.ĢD ROESY (ROtating frame noESY) for detecting/measuring 1H-1H through-space contacts and chemical exchange leveraging rotating-frame magnetization, indirect TD fixed at 128 increments, default mixing time is 0.6 sec. With positive diagonal peaks, negative crosspeaks indicate NOE and/or chemical exchange. Sophisticated pulse sequence elements reduce DQCOSY-like +/- zero-quantum coherence (ZQC) artifacts. Gives good signal on 10 mg samples.ĢD NOESY (Nuclear Overhauser Effect SpectroscopY) for detecting/measuring 1H-1H through-space contacts and chemical exchange, indirect TD fixed at 256 increments, default mixing time D9 = 0.6 sec (but can be modified). Gives very good signal levels on samples less than 10 mg.ĢD 1H-13C HMBC (Heteronuclear Multiple Bond Correlation), indirect TD fixed at 256 increments, filters eliminate one-bond correlations. When properly phased, CH and CH3 13C’s are positive, CH2’s are negative.ĢD COSY (COrrelation SpetroscopY), Indirect TD fixed at 200 increments.ĢD 1H-13C HSQC (Heteronuclear Single Quantum Coherence), indirect TD fixed at 128 increments multiplicity-edited so CH and CH3 peaks are positive and CH2 are negative (as in a DEPT135 spectrum), sensitivity improved over standard. Only 13C atoms with 1H’s attached are observed. 13C-detected, but signal enhanced via 1H magnetization.
Change NS, but not D1, AQ, or P1, for increased signal.ġD 13C DEPT135. For all multi-scan 1H 1D spectra with more scans, use these parameters and change NS.ġD 13C, 128 scans. Parameter SetġD 1H, single scan with 90° excitation, 17 sec delay to allow for full relaxation.ġD 1H, 8 scans, optimized for maximum signal and reliable integrals. Experiment times do not include overhead for sample changing, tuning, locking, shimming, or setting gain.
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